NMR pulses in structure research

To date, the available high field NMR instruments give the possibility of aquiring spectral information on solution state structures of macromolecules (e.g. proteins) as large as 30–60 kDa. With this technical background, NMR spectroscopy heavily contributes to relatively new research fields, such as biotechnology, structural biochemistry, molecular biology, virology etc.. This course, which does not require any previous knowledge of NMR spectroscopy aims to introduce the basic phenomena of solution state NMR spectroscopy. The goal is to get familiar with the theoretical and practical aspects of 1D-, 2D and nD-experiments useful for structure elucidation. First, the vector model is introduced followed by the description of the product operator formalism. (Sounds scary, but in reality it is just high school vector algebra.) Having these tools in hand, complex pulse-sequences such as COSY, TOCSY, NOESY, HSQC, HNCA etc. are explained and understood. During this course additional aspects of modern NMR spectroscopy will be outlined, such as relaxation, data processing, signal assignment strategies and structure calculation. Do not hesitate to come: this will be a lifetime experience, you will like it! (Language is optional, English or Hungarian upon demand.)